HPVScreen HR15

High-Risk Human Papillomavirus Detection by Multiplex qPCR

HPVScreen HR15 is a precision-engineered HPV DNA Genotyping PCR Kit designed for the qualitative detection of 15 high-risk human papillomavirus genotypes in female cervical samples. Based on TaqMan® multiplex real-time PCR chemistry, the kit delivers individual genotype resolution for HPV16 (ROX channel) and HPV18 (HEX channel) — the two genotypes responsible for the majority of HPV-associated cervical cancers — alongside collective detection of 13 further high-risk types via the FAM channel, all within a single reaction.

Sample integrity is confirmed in every run through co-amplification of the endogenous human RNaseP gene (Cy5 channel), eliminating the need for a separate extraction control. As a platform-agnostic HPV DNA Genotyping PCR Kit, HPVScreen HR15 is validated on all major real-time PCR instruments with four fluorescence channels and is available in 48-test and 96-test formats to suit varying laboratory throughput requirements.

<90 min

Sample to result turnaround time

10 copies/rxn

Analytical sensitivity (limit of detection)

15 genotypes

High-risk genotypes detected in a single reaction

4 channels

Fluorescence channels — FAM, HEX, ROX, Cy5 (IC)

Workflow Overview

Cervical Sample

VTM / swab

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DNA Extraction

200 µL input

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PCR Setup

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Amplification

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Result

Total turnaround time < 90 minutes

Technical Specifications

TechnologyTaqMan® multiplex qPCR

Limit of detection10 copies / reaction

Anti-contaminationdUTP-UNG system

Formats available48 or 96 tests/kit

Storage−20 ± 5 °C (dark)

Detection Channel Map

Channel	Analyte	Genotypes
FAM	HR Pool	31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68
HEX	HPV 18	Individual differentiation
ROX	HPV 16	Individual differentiation
Cy5	RNaseP IC	Endogenous internal control — sample integrity

Covered Genotypes

HPV 16 HPV 18 HPV 31 HPV 33 HPV 35 HPV 39 HPV 45 HPV 51 HPV 52 HPV 53 HPV 56 HPV 58 HPV 59 HPV 66 HPV 68 RNaseP (IC)

Individual differentiation Collectively reported (FAM channel) Internal control

Platform Compatibility

Bio-Rad CFX96

ABI 7500

Roche LC 480

Thermo QuantStudio 5

Qiagen RotorGene Q

Compatible with any real-time PCR instrument with ≥4 fluorescence channels (FAM · HEX/VIC · ROX · Cy5).

RNaseP Internal Control

Human endogenous RNaseP gene (Cy5 channel) is co-amplified in every reaction to confirm adequate sample cellularity and successful nucleic acid extraction.

Ordering Information

Catalogue Number	Description	Format	Storage
MOLHPVS48	HPVScreen HR15 Realtime PCR Assay	48 tests / kit	−20 ± 5 °C
MOLHPVS96	HPVScreen HR15 Realtime PCR Assay	96 tests / kit	−20 ± 5 °C

For Research Use Only (RUO). This product is not intended for use in diagnostic procedures. The assay is intended solely for investigational or research purposes and should be used by qualified personnel in appropriately equipped laboratories following good laboratory practice. Test results cannot be used alone as a basis for confirming or excluding clinical cases. Molsera Lifesciences Pvt Ltd, Sector 36, Noida, Uttar Pradesh.